Parallel Reaction Monitoring (PRM) Targeted Proteome
Parallel reaction monitoring (PRM) is an ion monitoring technique based on high-resolution and high-precision mass spectrometry. The principle of this technique is comparable to SRM/MRM, but it is more convenient in assay development for absolute quantification of proteins and peptides. It is most suitable for quantification of multiple proteins in complex sample with an attomole-level detection.
Advantages
High sensitivity;
High throughput;
Easier to develop assay.
Application
Verification of TMT differential protein;
Verification of label-free differential protein;
Protein and peptide absolute quantification;
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